PA5-118047

antibody from Invitrogen Antibodies

Targeting: MAP2K3 MAPKK3, MEK3, MKK3, PRKMK3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118047

Provider

Invitrogen Antibodies

Product name

MEK3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of MEK3 in Lane A: Jurkat Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-118047) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 39 kDa, Observed band size: 39 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MEK3 Polyclonal Antibody (Product # PA5-118047) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 39 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of MEK3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with MEK3 Polyclonal Antibody (Product # PA5-118047, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MEK3 in human kidney with MEK3 Polyclonal Antibody (Product # PA5-118047, 1:1,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MEK3 in human skeletal muscle with MEK3 Polyclonal Antibody (Product # PA5-118047, 1:1,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MEK3 in mouse kidney with MEK3 Polyclonal Antibody (Product # PA5-118047, 1:1,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MEK3 in mouse skeletal muscle with MEK3 Polyclonal Antibody (Product # PA5-118047, 1:1,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of MEK3 in Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate, Lane C: 0.5 mg HeLa Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118047) with a dilution of 1:100 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 39 kDa , Observed band size : 39 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MEK3 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate, Lane C: 0.5 mg HeLa Whole Cell Lysate 2 µL with MEK3 Polyclonal Antibody (Product # PA5-118047) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: MEK3 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 39 kDa.