TA502189

antibody from OriGene

Targeting: MAPK7 BMK1, ERK5, PRKM7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502189

Provider

OriGene

Proper citation

OriGene Cat#TA502189, RRID:AB_11124425

Product name

MAPK7 (ERK5) mouse monoclonal antibody, clone OTI5B9 (formerly 5B9)

Antibody type

Monoclonal

Description

MAPK7 (ERK5) mouse monoclonal antibody, clone OTI5B9 (formerly 5B9)

Host

Mouse

Conjugate

Unconjugated

Epitope

MAPK7

Isotype

IgG

Antibody clone number

OTI5B9

Vial size

100 µl

Concentration

0.79 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK7 monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK7 (RC203506, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK7.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-MAPK7 mouse monoclonal antibody (TA502189) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC203506 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MAPK7 antibody(TA502189), and then analyzed by flow cytometry.

Validation comment

FC