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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-110855 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YITRNEMLDI VDAIYQMVGN TVELPEEENT PEKRVDRIFA MMDKNADGKL TLQEFQEGSK ADPSIVQALS LYDGLV
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NCS1 in Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10, Lane 2: Human Cerebral Cortex tissue using NCS1 Polyclonal Antibody (Product # PA5-110855).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NCS1 in Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10, Lane 2: Mouse Cerebral Cortex tissue using NCS1 Polyclonal Antibody (Product # PA5-110855).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NCS1 in control (vector only transfected HEK293T lysate) and NCS1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402303) using NCS1 Polyclonal Antibody (Product # PA5-110855).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NCS1 in human cell line U-2 OS using NCS1 Polyclonal Antibody (Product # PA5-110855) shows localization to plasma membrane and vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCS1 in human cerebral cortex and liver tissues using NCS1 Polyclonal Antibody (Product # PA5-110855). Corresponding NCS1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCS1 in human liver tissue using NCS1 Polyclonal Antibody (Product # PA5-110855). Staining shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCS1 in human cerebral cortex tissue using NCS1 Polyclonal Antibody (Product # PA5-110855). Staining shows high expression.
