- GTX101803 - GeneTex ENO1 antibody

Submitted references Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.

Chang SJ, Liao EC, Yeo HY, Kuo WH, Chen HY, Tsai YT, Wei YS, Chen YJ, Wang YS, Li JM, Shih CC, Chan CH, Lai ZY, Chou HC, Chuang YJ, Chan HL
Archives of biochemistry and biophysics 2018 Jun 1;647:10-32

Identification of Differentiation-Related Proteins in Gastric Adenocarcinoma Tissues by Proteomics.

Zhou X, Yao K, Zhang L, Zhang Y, Han Y, Liu HL, Liu XW, Su G, Yuan WZ, Wei XD, Guan QL, Zhu BD
Technology in cancer research & treatment 2016 Oct;15(5):697-706

Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells.

Lin LL, Hsia CR, Hsu CL, Huang HC, Juan HF
BMC genomics 2015 Feb 5;16:41

Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.

Wu YH, Hu CW, Chien CW, Chen YJ, Huang HC, Juan HF
PloS one 2013;8(8):e70642

Surface α-enolase promotes extracellular matrix degradation and tumor metastasis and represents a new therapeutic target.

Hsiao KC, Shih NY, Fang HL, Huang TS, Kuo CC, Chu PY, Hung YM, Chou SW, Yang YY, Chang GC, Liu KJ
PloS one 2013;8(7):e69354

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: <50%
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 39799

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ?g of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX101803 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: A431 (GTX27909) B: H1299 10% SDS PAGE GTX101803 diluted at 1:3000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ENO1 antibody [N3C3] detects ENO1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ENO1 protein stained by ENO1 antibody [N3C3] (GTX101803) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human gastric tissue, using ENO1(GTX101803) antibody at 1:100 dilution.

GTX101803

Antibody data