Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA324847 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal ENO1 Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal ENO1 Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ENO1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ENO1 Antibody (Center) (Cat. #TA324847) western blot analysis in Jurkat,MCF-7,U251,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of C2C12 cell stained with ENO1 Antibody (Center)(Cat#TA324847).C2C12 cells were incubated with ENO1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . ENO1 immunoreactivity is localized to Cytoplasm significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lymph reacted with ENO1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ENO1 Antibody (Center) (Cat. #TA324847) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
