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LearnH00002023-M01
antibody from Novus Biologicals
Targeting: ENO1
ENO1L1, MBP-1, MPB1, PPH
Western blot ELISA Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Functional assayAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00002023-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002023-M01, RRID:AB_538563
- Product name
- Mouse Monoclonal Enolase 1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ENO1 - enolase 1, (alpha)
- Reactivity
- Human, Feline
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Protein arginine methyltransferase 5 mediates enolase-1 cell surface trafficking in human lung adenocarcinoma cells.
Enolase-1 expression in the stratum corneum is elevated with parakeratosis of atopic dermatitis and disrupts the cellular tight junction barrier in keratinocytes.
The interaction of enolase-1 with caveolae-associated proteins regulates its subcellular localization.
Overexpression of α-enolase correlates with poor survival in canine mammary carcinoma.
Systematic proteomic analysis of human hepotacellular carcinoma cells reveals molecular pathways and networks involved in metastasis.
Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor.
Comparative proteomics analysis of Barrett metaplasia and esophageal adenocarcinoma using two-dimensional liquid mass mapping.
Zakrzewicz D, Didiasova M, Krüger M, Giaimo BD, Borggrefe T, Mieth M, Hocke AC, Zakrzewicz A, Schaefer L, Preissner KT, Wygrecka M
Biochimica et biophysica acta. Molecular basis of disease 2018 May;1864(5 Pt A):1816-1827
Biochimica et biophysica acta. Molecular basis of disease 2018 May;1864(5 Pt A):1816-1827
Enolase-1 expression in the stratum corneum is elevated with parakeratosis of atopic dermatitis and disrupts the cellular tight junction barrier in keratinocytes.
Tohgasaki T, Ozawa N, Yoshino T, Ishiwatari S, Matsukuma S, Yanagi S, Fukuda H
International journal of cosmetic science 2018 Apr;40(2):178-186
International journal of cosmetic science 2018 Apr;40(2):178-186
The interaction of enolase-1 with caveolae-associated proteins regulates its subcellular localization.
Zakrzewicz D, Didiasova M, Zakrzewicz A, Hocke AC, Uhle F, Markart P, Preissner KT, Wygrecka M
The Biochemical journal 2014 Jun 1;460(2):295-307
The Biochemical journal 2014 Jun 1;460(2):295-307
Overexpression of α-enolase correlates with poor survival in canine mammary carcinoma.
Chu PY, Hsu NC, Liao AT, Shih NY, Hou MF, Liu CH
BMC veterinary research 2011 Oct 21;7:62
BMC veterinary research 2011 Oct 21;7:62
Systematic proteomic analysis of human hepotacellular carcinoma cells reveals molecular pathways and networks involved in metastasis.
Yu Y, Shen H, Yu H, Zhong F, Zhang Y, Zhang C, Zhao J, Li H, Chen J, Liu Y, Yang P
Molecular bioSystems 2011 Jun;7(6):1908-16
Molecular bioSystems 2011 Jun;7(6):1908-16
Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor.
Ho JA, Chang HC, Shih NY, Wu LC, Chang YF, Chen CC, Chou C
Analytical chemistry 2010 Jul 15;82(14):5944-50
Analytical chemistry 2010 Jul 15;82(14):5944-50
Comparative proteomics analysis of Barrett metaplasia and esophageal adenocarcinoma using two-dimensional liquid mass mapping.
Zhao J, Chang AC, Li C, Shedden KA, Thomas DG, Misek DE, Manoharan AP, Giordano TJ, Beer DG, Lubman DM
Molecular & cellular proteomics : MCP 2007 Jun;6(6):987-99
Molecular & cellular proteomics : MCP 2007 Jun;6(6):987-99
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody (M01), clone 8G8.Lane 1: ENO1 transfected lysate(47 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - ENO1 monoclonal antibody (M01), clone 8G8. Analysis of ENO1 expression in human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - ENO1 monoclonal antibody (M01), clone 8G8 Analysis of ENO1 expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Enolase 1 Antibody (8G8) [H00002023-M01] - Detection limit for recombinant GST tagged ENO1 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1 ug/ml.
