MA1-22700

antibody from Invitrogen Antibodies

Targeting: TNNI3 CMD2A, CMH7, TNNC1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-22700

Provider

Invitrogen Antibodies

Product name

Cardiac Troponin I Monoclonal Antibody (19C7)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-22700 detects Cardiac Troponin 1 in Human, Mouse, Rat, Bovine, Feline, Canine, Fish, Porcine, Goat, and Rabbit samples.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Feline, Goat, Porcine, Rabbit

Host

Mouse

Isotype

IgG

Antibody clone number

19C7

Vial size

200 µg

Concentration

4.3 mg/mL

Storage

4° C, do not freeze

References

Submitted references

The Protective Role of Yin-Yang 1 in Cardiac Injury and Remodeling After Myocardial Infarction.

Huang Y, Li L, Chen H, Liao Q, Yang X, Yang D, Xia X, Wang H, Wang WE, Chen L, Zeng C
Journal of the American Heart Association 2021 Nov 2;10(21):e021895

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End-diastolic force pre-activates cardiomyocytes and determines contractile force: role of titin and calcium.

Najafi A, van de Locht M, Schuldt M, Schönleitner P, van Willigenburg M, Bollen I, Goebel M, Ottenheijm CAC, van der Velden J, Helmes M, Kuster DWD
The Journal of physiology 2019 Sep;597(17):4521-4531

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Cardiomyocyte Hypocontractility and Reduced Myofibril Density in End-Stage Pediatric Cardiomyopathy.

Bollen IAE, van der Meulen M, de Goede K, Kuster DWD, Dalinghaus M, van der Velden J
Frontiers in physiology 2017;8:1103

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Genotype-specific pathogenic effects in human dilated cardiomyopathy.

Bollen IAE, Schuldt M, Harakalova M, Vink A, Asselbergs FW, Pinto JR, Krüger M, Kuster DWD, van der Velden J
The Journal of physiology 2017 Jul 15;595(14):4677-4693

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Liver (Lane 3), Mouse Skeletal Muscle (Lane 4) and Mouse Brain (Lane 5). The blot was probed with Anti-Cardiac Troponin I Monoclonal Antibody (19C7) (Product # MA1-22700, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 24 kDa band corresponding to Cardiac Troponin I was detected only in Mouse and Rat Heart which are reported to be positive for Cardiac Troponin I expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Liver (Lane 3), Mouse Skeletal Muscle (Lane 4) and Mouse Brain (Lane 5). The blot was probed with Anti-Cardiac Troponin I Monoclonal Antibody (19C7) (Product # MA1-22700, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 24 kDa band corresponding to Cardiac Troponin I was detected only in Mouse and Rat Heart which are reported to be positive for Cardiac Troponin I expression.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Protein analysis in isolated cardiomyocytes from WT and HET hearts A , in HET cardiomyocytes a longer titin isoform is seen. B , Phos-tag analysis of cardiac troponin I was performed to assess its phosphorylation state. No difference in cTnI phosphorylation was seen between WT ( N = 6) and HET ( N = 5). C , immunoblot analysis was performed to measure PLN expression levels and phosphorylation. D and E , no difference was seen in expression levels ( D ) and phosphorylation at Ser16 ( E ) between WT ( N = 6) and HET ( N = 5). Data are shown as means +- SEM.