AM06710PU-N

antibody from OriGene

Targeting: TNNI2 AMCD2B, DA2B, FSSV

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM06710PU-N

Provider

OriGene

Product name

Troponin I fast skeletal muscle (TNNI2) mouse monoclonal antibody, clone 2F12G2, Purified

Antibody type

Monoclonal

Description

Troponin I fast skeletal muscle (TNNI2) mouse monoclonal antibody, clone 2F12G2, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

TNNI2

Isotype

IgG

Antibody clone number

2F12G2

Vial size

100 µg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis using TNNI2 mAb against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 21 kDa)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis using TNNI2 mAb against HEK293 (1) and TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

Validation comment

ELISA

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence analysis of Hela cells using TNNI2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric analysis of NIH/3T3 cells using TNNI2 mouse mAb (green) and negative control (red).

Validation comment

FC