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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- TA506926 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA506926, RRID:AB_2623989
- Product name
- SERPINB3 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)
- Antibody type
- Monoclonal
- Description
- SERPINB3 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SERPINB3
- Isotype
- IgG
- Antibody clone number
- OTI1F10
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB3 (RC202683, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB3.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Standard curve for ELISA analysis with SCCA (SCCA1+SCCA2) recombinant protein (dilution range from 100pg/ml to 10ug/ml) using SCCA Capture Antibody (Cat# TA506926) at 5ug/ml and HRP conjugated SCCA Detection mAb (Cat# TA506973) at 0.025ug/ml.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SERPINB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506926)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SERPINB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506926)
- Validation comment
- IHC
