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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30164 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SERPINB3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299.
- Concentration
- 0.57 mg/mL
Submitted references Hepatocyte-Specific Deletion of HIF2α Prevents NASH-Related Liver Carcinogenesis by Decreasing Cancer Cell Proliferation.
SerpinB3 drives cancer stem cell survival in glioblastoma.
Foglia B, Sutti S, Cannito S, Rosso C, Maggiora M, Autelli R, Novo E, Bocca C, Villano G, Ramavath NN, Younes R, Tusa I, Rovida E, Pontisso P, Bugianesi E, Albano E, Parola M
Cellular and molecular gastroenterology and hepatology 2022;13(2):459-482
Cellular and molecular gastroenterology and hepatology 2022;13(2):459-482
SerpinB3 drives cancer stem cell survival in glioblastoma.
Lauko A, Volovetz J, Turaga SM, Bayik D, Silver DJ, Mitchell K, Mulkearns-Hubert EE, Watson DC, Desai K, Midha M, Hao J, McCortney K, Steffens A, Naik U, Ahluwalia MS, Bao S, Horbinski C, Yu JS, Lathia JD
Cell reports 2022 Sep 13;40(11):111348
Cell reports 2022 Sep 13;40(11):111348
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SerpinB3 using 30 µg of HCT116 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SerpinB3 polyclonal antibody (Product # PA5-30164) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SERPINB3 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a SERPINB3 Polyclonal Antibody (Product # PA5-30164) at a dilution of 1:3000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SerpinB3 in methanol-fixed A549 cells using a SerpinB3 polyclonal antibody (Product # PA5-30164) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SERPINB3 was performed in paraffin-embedded human cervical carcinoma tissue using SERPINB3 Polyclonal Antibody (Product # PA5-30164) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
