A305-705A-T

antibody from Invitrogen Antibodies

Targeting: TTYH3 KIAA1691

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A305-705A-T

Provider

Invitrogen Antibodies

Product name

TTYH3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TTYH3 by western blot. Samples: Whole cell lysate (50 µg) from NCI-H226, HEK293T, HeLa, Jurkat, and Hs 294T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TTYH3 antibody (Product # A305-705A-T; lot 2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TTYH3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Malme-3M cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TTYH3 antibody A305-705A-M (lot A305-705A-M-1) used for IP at 50 µl per reaction. TTYH3 was also immunoprecipitated by rabbit anti-TTYH3 antibody BL22705. For blotting immunoprecipitated TTYH3, A305-705A-M was used at 1:100. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TTYH3 by western blot. Samples: Whole cell lysate (15 µg) from MALME-3M, HeLa, and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TTYH3 antibody A305-705A-M (lot A305-705A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.