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Learn17534-1-AP
antibody from Proteintech Group
Targeting: IFT52
C20orf9, CGI-53, dJ1028D15.1, NGD2, NGD5
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- 17534-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#17534-1-AP, RRID:AB_2280005
- Product name
- IFT52 antibody
- Antibody type
- Polyclonal
- Description
- IFT52 antibody (Cat. #17534-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, Canine and has been validated for the following applications: IF, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Homozygous mutations in CCDC34 cause male infertility with oligoasthenoteratozoospermia in humans and mice.
Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.
IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.
Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat.
Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice.
Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.
Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice.
Inhibition of Hedgehog signaling suppresses proliferation and microcyst formation of human Autosomal Dominant Polycystic Kidney Disease cells.
FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.
Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.
IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.
Destabilization of the IFT-B cilia core complex due to mutations in IFT81 causes a Spectrum of Short-Rib Polydactyly Syndrome.
IFT52 mutations destabilize anterograde complex assembly, disrupt ciliogenesis and result in short rib polydactyly syndrome.
Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.
Active transport and diffusion barriers restrict Joubert Syndrome-associated ARL13B/ARL-13 to an Inv-like ciliary membrane subdomain.
The cilia protein IFT88 is required for spindle orientation in mitosis.
Cong J, Wang X, Amiri-Yekta A, Wang L, Kherraf ZE, Liu C, Cazin C, Tang S, Hosseini SH, Tian S, Daneshipour A, Wang J, Zhou Y, Zeng Y, Yang S, He X, Li J, Cao Y, Jin L, Ray PF, Zhang F
Journal of medical genetics 2021 Aug 4;
Journal of medical genetics 2021 Aug 4;
Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.
Wang W, Allard BA, Pottorf TS, Wang HH, Vivian JL, Tran PV
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 May;34(5):6369-6381
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 May;34(5):6369-6381
IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.
Vitre B, Taulet N, Guesdon A, Douanier A, Dosdane A, Cisneros M, Maurin J, Hettinger S, Anguille C, Taschner M, Lorentzen E, Delaval B
EMBO reports 2020 Jun 4;21(6):e49234
EMBO reports 2020 Jun 4;21(6):e49234
Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat.
Zhang Y, Chen Y, Zheng J, Wang J, Duan S, Zhang W, Yan X, Zhu X
Journal of molecular cell biology 2019 May 1;11(5):383-394
Journal of molecular cell biology 2019 May 1;11(5):383-394
Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice.
Liu C, He X, Liu W, Yang S, Wang L, Li W, Wu H, Tang S, Ni X, Wang J, Gao Y, Tian S, Zhang L, Cong J, Zhang Z, Tan Q, Zhang J, Li H, Zhong Y, Lv M, Li J, Jin L, Cao Y, Zhang F
American journal of human genetics 2019 Dec 5;105(6):1168-1181
American journal of human genetics 2019 Dec 5;105(6):1168-1181
Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.
Dupont MA, Humbert C, Huber C, Siour Q, Guerrera IC, Jung V, Christensen A, Pouliet A, Garfa-Traoré M, Nitschké P, Injeyan M, Millar K, Chitayat D, Shannon P, Girisha KM, Shukla A, Mechler C, Lorentzen E, Benmerah A, Cormier-Daire V, Jeanpierre C, Saunier S, Delous M
Human molecular genetics 2019 Aug 15;28(16):2720-2737
Human molecular genetics 2019 Aug 15;28(16):2720-2737
Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice.
Liu W, He X, Yang S, Zouari R, Wang J, Wu H, Kherraf ZE, Liu C, Coutton C, Zhao R, Tang D, Tang S, Lv M, Fang Y, Li W, Li H, Zhao J, Wang X, Zhao S, Zhang J, Arnoult C, Jin L, Zhang Z, Ray PF, Cao Y, Zhang F
American journal of human genetics 2019 Apr 4;104(4):738-748
American journal of human genetics 2019 Apr 4;104(4):738-748
Inhibition of Hedgehog signaling suppresses proliferation and microcyst formation of human Autosomal Dominant Polycystic Kidney Disease cells.
Silva LM, Jacobs DT, Allard BA, Fields TA, Sharma M, Wallace DP, Tran PV
Scientific reports 2018 Mar 21;8(1):4985
Scientific reports 2018 Mar 21;8(1):4985
FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.
Honda A, Kita T, Seshadri SV, Misaki K, Ahmed Z, Ladbury JE, Richardson GP, Yonemura S, Ladher RK
Proceedings of the National Academy of Sciences of the United States of America 2018 Aug 14;115(33):8388-8393
Proceedings of the National Academy of Sciences of the United States of America 2018 Aug 14;115(33):8388-8393
Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.
Takei R, Katoh Y, Nakayama K
Biology open 2018 Apr 30;7(5)
Biology open 2018 Apr 30;7(5)
IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.
Taulet N, Vitre B, Anguille C, Douanier A, Rocancourt M, Taschner M, Lorentzen E, Echard A, Delaval B
Nature communications 2017 Dec 4;8(1):1928
Nature communications 2017 Dec 4;8(1):1928
Destabilization of the IFT-B cilia core complex due to mutations in IFT81 causes a Spectrum of Short-Rib Polydactyly Syndrome.
Duran I, Taylor SP, Zhang W, Martin J, Forlenza KN, Spiro RP, Nickerson DA, Bamshad M, Cohn DH, Krakow D
Scientific reports 2016 Sep 26;6:34232
Scientific reports 2016 Sep 26;6:34232
IFT52 mutations destabilize anterograde complex assembly, disrupt ciliogenesis and result in short rib polydactyly syndrome.
Zhang W, Taylor SP, Nevarez L, Lachman RS, Nickerson DA, Bamshad M, University of Washington Center for Mendelian Genomics Consortium., Krakow D, Cohn DH
Human molecular genetics 2016 Sep 15;25(18):4012-4020
Human molecular genetics 2016 Sep 15;25(18):4012-4020
Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.
Xu Y, Cao J, Huang S, Feng D, Zhang W, Zhu X, Yan X
PloS one 2015;10(4):e0124378
PloS one 2015;10(4):e0124378
Active transport and diffusion barriers restrict Joubert Syndrome-associated ARL13B/ARL-13 to an Inv-like ciliary membrane subdomain.
Cevik S, Sanders AA, Van Wijk E, Boldt K, Clarke L, van Reeuwijk J, Hori Y, Horn N, Hetterschijt L, Wdowicz A, Mullins A, Kida K, Kaplan OI, van Beersum SE, Man Wu K, Letteboer SJ, Mans DA, Katada T, Kontani K, Ueffing M, Roepman R, Kremer H, Blacque OE
PLoS genetics 2013;9(12):e1003977
PLoS genetics 2013;9(12):e1003977
The cilia protein IFT88 is required for spindle orientation in mitosis.
Delaval B, Bright A, Lawson ND, Doxsey S
Nature cell biology 2011 Apr;13(4):461-8
Nature cell biology 2011 Apr;13(4):461-8
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human testis tissue were subjected to SDS PAGE followed by western blot with 17534-1-AP(IFT52 antibody) at dilution of 1:400
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using IFT52 antibody 17534-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP result of anti-IFT52 (17534-1-AP for IP and Detection) with HeLa cell lysate.
- Sample type
- cell line
