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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- GTX83972 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83972, RRID:AB_10734267
- Product name
- NXNL2 antibody [2B11]
- Antibody type
- Monoclonal
- Reactivity
- Human, Porcine, Simian
- Host
- Mouse
Submitted references Two-Step Reactivation of Dormant Cones in Retinitis Pigmentosa.
Wang W, Lee SJ, Scott PA, Lu X, Emery D, Liu Y, Ezashi T, Roberts MR, Ross JW, Kaplan HJ, Dean DC
Cell reports 2016 Apr 12;15(2):372-85
Cell reports 2016 Apr 12;15(2):372-85
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NXNL2 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NXNL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NXNL2.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-NXNL2 mouse monoclonal antibody (GTX83972).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NXNL2 mouse monoclonal antibody. (GTX83972, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-NXNL2 mouse monoclonal antibody. (GTX83972, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-NXNL2 mouse monoclonal antibody. (GTX83972, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-NXNL2 mouse monoclonal antibody. (GTX83972, Dilution 1:50)
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-NXNL2 antibody(GTX83972),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
