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LearnPA5-109235
antibody from Invitrogen Antibodies
Targeting: CYP19A1
ARO, ARO1, aromatase, CPV1, CYAR, CYP19, P-450AROM
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-109235 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aromatase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: HepG2, SH-SY-5Y, SK-Br-3, human placenta tissue, mouse cerebellum tissue lysate.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Aromatase in mouse cerebellum tissue lysate. Samples were incubated with Aromatase polyclonal antibody (Product # PA5-109235), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Aromatase in SK-Br-3 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Aromatase polyclonal antibody (Product # PA5-109235), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Aromatase in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Aromatase polyclonal antibody (Product # PA5-109235), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Aromatase in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Aromatase polyclonal antibody (Product # PA5-109235), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Aromatase in paraffin-embedded human placenta tissue. Samples were incubated with Aromatase polyclonal antibody (Product # PA5-109235), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Aromatase in HepG2 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Aromatase polyclonal antibody (Product # PA5-109235) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
