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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunocytochemistry [4]
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Validation data
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- Product number
- ALX-804-291-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-291-C100, RRID:AB_2050969
- Product name
- Cathepsin L/procathepsin L monoclonal antibody (CPLH 33/2)
- Antibody type
- Monoclonal
- Antigen
- Purified from natural sources
- Description
- Protein G-affinity purified.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CPLH 33/2
- Vial size
- 100 μg
- Storage
- +4°C
- Handling
- Do not freeze.
Submitted references Immunohistochemical and clinical evaluation of cathepsin expression in soft tissue sarcomas.
Monoclonal antibodies against cathepsin L and procathepsin L of different species.
Hybridoma cells producing antibodies to cathepsin L have greatly reduced potential for tumour growth.
Würl P, Taubert H, Meye A, Dansranjavin T, Weber E, Günther D, Berger D, Schmidt H, Dralle H, Rath FW
Virchows Archiv : an international journal of pathology 1997 Mar;430(3):221-5
Virchows Archiv : an international journal of pathology 1997 Mar;430(3):221-5
Monoclonal antibodies against cathepsin L and procathepsin L of different species.
Weber E, Bahn H, Günther D
Hybridoma 1997 Apr;16(2):159-66
Hybridoma 1997 Apr;16(2):159-66
Hybridoma cells producing antibodies to cathepsin L have greatly reduced potential for tumour growth.
Weber E, Günther D, Laube F, Wiederanders B, Kirschke H
Journal of cancer research and clinical oncology 1994;120(9):564-7
Journal of cancer research and clinical oncology 1994;120(9):564-7
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot (ECL) of purified human cathepsin L (100ng per lane). Antibody dilutions: Lane 1: 1:20'000; Lane 2: 1:50'000; Lane 3: 1:100'000; Lane 4: 1:200'000.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot (ECL) of human cathepsin L in kidney parenchyma homogenates. Antibody dilutions: Lane 1: 1:10'000; Lane 2: 1:25'000; Lane 3: 1:50'000; Lane 4: 1:100'000.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot (NBT) of human cathepsin L in kidney parenchyma homogenates. Antibody dilutions: Lane 1: 1:10'000; Lane 2: 1:25'000; Lane 3: 1:50'000; Lane 4: 1:100'000.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunostaining with MAb to Cathepsin L (CPLH 33/2) of normal human lung epithelium (A) and alveolar macrophages (B). Courtesy of Dr. F. Buehling, Magdeburg
