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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 25182-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYH1 (N-terminal) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSSDSEMAI FGEAAPFLRK SERERIEAQN KPFDAKTSVF VVDPKESFVK ATVQSREGGK VTAKTEAGAT VTVKDDQVFP MNPPKYDKIE DMAMMTHLHE PAVLYNLKER YAAWMIYTYS GLFCVTVNPY KWLPVYNAEV VTAYRGKKRQ EAPPHIFSIS DNAYQFMLTD RENQSILITG ESGAGKTVNT KRVIQYFATI AVTGEKKKEE VTSGKMQGTL EDQIISANPL LEAFGNAKTV RNDNSSRFGK FIRIHFGTTG KLASADIETY LLEKSRVTFQ LKAERSYHIF YQIMSNKKPD LIEMLLITTN PYDYAFVSQG EITVPSIDDQ EELMATDSAI EILGFTSDER V (1-350 aa encoded by BC114545)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 25182-1-AP ( MYH1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 25182-1-AP ( MYH1 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 25182-1-AP ( MYH1 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-MYH1 (IP:25182-1-AP, 5ug; Detection:25182-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.
