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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 12387-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RGMA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SAVHGIEDL MSQHNCSKDG PTSQPRLRTL PPAGDSQERS DSPEICHYEK SFHKHSATPN YTHCGLFGDP HLRTFTDRFQ TCKVQGAWPL IDNNYLNVQV TNTPVLPGSA ATATSKLTII FKNFQECVDQ KVYQAEMDEL PAAFVDGSKN GGDKHGANSL KITEKVSGQH VEIQAKYIGT TIVVRQVGRY LTFAVRMPEE VVNAVEDWDS QGLYLCLRGC PLNQQIDFQA FHTNAEGTGA RRLAAASPAP TAPETFPYET AVAKCKEKLP VEDLYYQACV FDLLTTGDVN FTLAAYYALE DVKMLHSNKD KLHLYERTRD LPGRAAAGLP LAPRPLLGAL VPLLALLPVF C (136-485 aa encoded by BC015886)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.22 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12387-1-AP (RGMA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 12387-1-AP ( RGMA antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RGMA (IP:12387-1-AP, 4ug; Detection:12387-1-AP 1:400) with mouse testis tissue lysate 4000ug.
