Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-34353 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM106B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human liver tissue lysate.
- Concentration
- 1 mg/mL
Submitted references Intracellular Proteolysis of Progranulin Generates Stable, Lysosomal Granulins that Are Haploinsufficient in Patients with Frontotemporal Dementia Caused by GRN Mutations.
Holler CJ, Taylor G, Deng Q, Kukar T
eNeuro 2017 Jul-Aug;4(4)
eNeuro 2017 Jul-Aug;4(4)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human liver tissue lysate using a TMEM106B polyclonal antibody (Product # PA5-34353) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TMEM106B in human brain tissue lysate with TMEM106B Polyclonal Antibody (Product # PA5-34353) at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TMEM106B in human liver tissue with TMEM106B Polyclonal Antibody (Product # PA5-34353) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TMEM106B in human liver tissue with TMEM106B Polyclonal Antibody (Product # PA5-34353) at 2.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Expression of recombinant human GRNs and identification of antibodies that detect GRNs. A , Schematic of human GRN expression constructs. Human GRN sequences (Table 2), with and without adjacent C-terminal linker regions, were synthesized to include the N-terminal PGRN signal peptide (SP), followed by twin-Strep (SAWSHPQFEK) tags and a single FLAG (DYKDDDDK) tag. Throughout the manuscript, individual GRNs are referred to by their numerical sequential designation (i.e., GRN-1, GRN-2, etc.), which correspond to their original alphabetical designation (i.e., GRN-G, GRN-F, etc.). B , C , HEK293T cells were transfected with the human GRN constructs and 48 h later either ( B ) whole-cell lysates or ( C ) conditioned media were analyzed by immunoblot for protein expression using the StrepMAB-immo antibody. D , Cell lysates from GRN(+linker) overexpressing cells were mock (-) or PNGase F (+) treated to detect glycosylated proteins. E , F , GRN-expressing HEK293T cell lysates were analyzed by immunoblot to identify PGRN antibodies that either ( E ) detect single linker regions of PGRN or ( F ) detect GRNs. The specific PGRN linker or GRN(s) detected are listed under each antibody. Detailed information about the PGRN antibodies screened can be found in Table 3. Asterisks (*) denote nonspecific protein bands.