F0300

antibody from MilliporeSigma / Merck KGaA

Targeting: FGFR2 BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Protein array (Recommended by provider)

Antibody Data

Product number

F0300

Provider

MilliporeSigma / Merck KGaA

Product name

Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Storage

Store at -20°C

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 87

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.