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LearnMAB684-100
antibody from R&D Systems
Targeting: FGFR2
BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB684-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human FGFR2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects alpha isoforms (3 Ig-like domains) and beta isoforms (2 Ig-like domains) of human FGF R2 in direct ELISAs and Western blots. In direct ELISAs, this antibody reacts with all isoforms of rhFGF R2 but shows approximately a 4-fold preference for (IIIc) isoforms. Approximately 100% cross-reactivity with recombinant mouse FGF R2, 15% cross-reactivity with recombinant human (rh) FGF RI ( beta isoforms), and no cross-reactivity with rhFGF RI ( alpha isoforms), rhFGF R3, or rhFGF R4 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 98725
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
Ponte AL, Marais E, Gallay N, Langonné A, Delorme B, Hérault O, Charbord P, Domenech J
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1737-45
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1737-45
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody. Recombinant Human FGF R2 alpha (IIIc) Fc Chimera (Catalog # 712-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2 alpha (IIIc) Fc Chimera (4 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 Monoclonal Antibody (Catalog # MAB684). The ND50 is typically 1-5 µg/mL in the presence of heparin (10 µg/mL).
