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LearnTA503137
antibody from OriGene
Targeting: FGFR2
BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Flow cytometry [2]
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Validation data
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- Product number
- TA503137 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503137, RRID:AB_11140739
- Product name
- FGFR2 mouse monoclonal antibody, clone OTI5C5 (formerly 5C5)
- Antibody type
- Monoclonal
- Description
- FGFR2 mouse monoclonal antibody, clone OTI5C5 (formerly 5C5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FGFR2
- Isotype
- IgG
- Antibody clone number
- OTI5C5
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references FGFR2 Promotes Gastric Cancer Progression by Inhibiting the Expression of Thrombospondin4 via PI3K-Akt-Mtor Pathway.
Huang T, Liu D, Wang Y, Li P, Sun L, Xiong H, Dai Y, Zou M, Yuan X, Qiu H
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;50(4):1332-1345
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;50(4):1332-1345
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (RC217098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-FGFR2 monoclonal antibody at 1:200.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- No Cross-Reactivity of anti-FGFR2 (TA503137) with FGFR1, FGFR3 and FGFR4.HEK293T cells were transfected with the DDK(FLAG)-tagged DNAs of FGFR2 (RC217098),FGFR1(RC202080),FGFR3(RC215533),FGFR4(RC204230) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2(TA503137).(1:500) and anti-DDK (1:2000), respectively.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-FGFR2 mouse monoclonal antibody (TA503137) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FGFR2(RC217098).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503137)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503137)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503137)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503137)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503137)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC217098 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FGFR2 antibody(TA503137), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-FGFR2 antibody(TA503137),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
