GTX85461

antibody from GeneTex

Targeting: NEFM NEF3, NF-M, NFM

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX85461

Provider

GeneTex

Proper citation

GeneTex Cat#GTX85461, RRID:AB_10629221

Product name

NF-M antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Chicken/Avian

References

Submitted references

Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.

Brügger V, Duman M, Bochud M, Münger E, Heller M, Ruff S, Jacob C
Nature communications 2017 Jan 31;8:14272

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HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.

Brügger V, Engler S, Pereira JA, Ruff S, Horn M, Welzl H, Münger E, Vaquié A, Sidiropoulos PN, Egger B, Yotovski P, Filgueira L, Somandin C, Lühmann TC, D'Antonio M, Yamaguchi T, Matthias P, Suter U, Jacob C
PLoS biology 2015;13(9):e1002258

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Localisation and activation of the neurokinin 1 receptor in the enteric nervous system of the mouse distal colon.

Pelayo JC, Veldhuis NA, Eriksson EM, Bunnett NW, Poole DP
Cell and tissue research 2014 May;356(2):319-32

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Dissociated cell culture prepared from an adult rat brain. NFM (green staining) stains neurites in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to co-stain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NF-M antibody detects NF-M protein at cytoskeleton by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NF-M protein stained by NF-M antibody (GTX85461) diluted at 1:250.Red: F-Actin , stained by F-Actin antibody [NH3] (GTX20205) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Dissociated cell culture prepared from an adult rat brain. NF-M (green staining) stains neurites in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining.