66050-1-IG

antibody from Invitrogen Antibodies

Targeting: NDUFA4L2 COXFA4L2, FLJ26118, NUOMS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

66050-1-IG

Provider

Invitrogen Antibodies

Product name

NDUFA4L2 Monoclonal Antibody (1G1H10)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MAGASLGAR FYRQIKRHPG IIPMIGLICL GMGSAALYLL RLALRSPDVC WDRKNNPEPW NRLSPNDQYK FLAVSTDYKK LKKDRPDF (1-87 aa encoded by BC011910)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1G1H10

Vial size

150 µL

Concentration

1.06 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-IG (NDUFA4L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NDUFA4L2 antibody (Product # 66050-1-IG, Clone: 1G1H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using NDUFA4L2 antibody 66050-1-IG at 1:25 dilution and Rhodamine-labeled goat anti-mouse IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using NDUFA4L2 antibody 66050-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66050-1-IG ( NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66050-1-IG ( NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).