- PA5-24816 - Invitrogen Antibodies COQ9 antibody

Submitted references The clinical heterogeneity of coenzyme Q10 deficiency results from genotypic differences in the Coq9 gene.

Luna-Sánchez M, Díaz-Casado E, Barca E, Tejada MÁ, Montilla-García Á, Cobos EJ, Escames G, Acuña-Castroviejo D, Quinzii CM, López LC
EMBO molecular medicine 2015 May;7(5):670-87

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis using a COQ9 polyclonal antibody (Product # PA5-24816) in mouse heart tissue lysates (35 µg per lane).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human lung tissue using a COQ9 polyclonal antibody (Product # PA5-24816), followed by HRP-conjugated secondary antibody and DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow cytometry analysis of NCI-H460 cells using a COQ9 polyclonal antibody (Product # PA5-24816) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 5 Levels of COQ biosynthetic proteins A-D Representative Western blot and quantitation of Western blot bands of COQ7 (A), ADCK3 (B), COQ5 (C) and COQ6 (D), and VDAC1 as a loading control in the kidneys of 3-month-old mice. * P < 0.05; ** P < 0.01; *** P < 0.001; Coq9 Q95X and Coq9 R239X mice versus Coq9 +/+ mice. ## P < 0.01; ### P < 0.001; Coq9 Q95X versus Coq9 R239X mice. One-way ANOVA with a Tukey's post hoc test. E-H Representative Western blot and quantitation of Western blot bands of COQ7 (E), ADCK3 (F), COQ5 (G) and COQ6 (H), and VDAC1 as a loading control in skeletal muscle of 3-month-old mice. ** P < 0.01; *** P < 0.001; Coq9 Q95X and Coq9 R239X mice versus Coq9 +/+ mice. # P < 0.05; ## P < 0.01; Coq9 Q95X versus Coq9 R239X mice. Data information: All values are presented as mean +- SD. One-way ANOVA with a Tukey's post hoc test. Numbers above columns indicate P -values of the one-way ANOVA test. Coq9 +/+ mice n = 4; Coq9 Q95X and Coq9 R239X mice n = 5. Source data are available online for this figure.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 Moderate CoQ deficiency in Coq9 Q95X mice leads to impaired mitochondrial bioenergetics function A-C Mitochondrial CoQ 9 levels from cerebrum (A), kidney (B) and skeletal muscle (C) of Coq9 +/+ and Coq9 Q95X males and females. n = 8 for each group. D-F CI+CIII activity in cerebrum (D), kidney (E) and skeletal muscle (F) of male and female Coq9 +/+ and Coq9 Q95X mice. n = 6 for each group. G-I CII+CIII activity in cerebrum (G), kidney (H) and skeletal muscle (I) of male and female Coq9 +/+ and Coq9 Q95X mice. n = 6 for each group. J-L Blue-native gel electrophoresis (BNGE) followed by immunoblotting analysis of mitochondrial supercomplexes from Coq9 +/+ ( n = 3) and Coq9 Q95X mice ( n = 4) at 3 months of age. Data information: (A-I) Data are expressed as mean +- SD. Statistical analyses were performed on Coq9 +/+ male mice versus Coq9 Q95X male mice and Coq9 +/+ female mice versus Coq9 Q95X female mice. ** P < 0.01; *** P < 0.001. Student's t -test. Complex I+III, NADH-cytochrome c reductase; complex II+III, SDH-cytochrome c reductase. Source data are available online for this figure.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Supplementary Figure S2

PA5-24816

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