Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- 20485-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20485-1-AP, RRID:AB_10695184
- Product name
- TMEM165 antibody
- Antibody type
- Polyclonal
- Description
- TMEM165 antibody (Cat. #20485-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.
The human Golgi protein TMEM165 transports calcium and manganese in yeast and bacterial cells.
TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity.
The Ca(2+)/H(+) antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca(2+) ATPase (SPCA1).
Nthiga TM, Shrestha BK, Bruun JA, Larsen KB, Lamark T, Johansen T
The Journal of cell biology 2021 Jun 7;220(6)
The Journal of cell biology 2021 Jun 7;220(6)
The human Golgi protein TMEM165 transports calcium and manganese in yeast and bacterial cells.
Stribny J, Thines L, Deschamps A, Goffin P, Morsomme P
The Journal of biological chemistry 2020 Mar 20;295(12):3865-3874
The Journal of biological chemistry 2020 Mar 20;295(12):3865-3874
TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity.
Lee JS, Kim MY, Park ER, Shen YN, Jeon JY, Cho EH, Park SH, Han CJ, Choi DW, Jang JJ, Suh KS, Hong J, Kim SB, Lee KH
Oncology reports 2018 Sep;40(3):1297-1306
Oncology reports 2018 Sep;40(3):1297-1306
The Ca(2+)/H(+) antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca(2+) ATPase (SPCA1).
Reinhardt TA, Lippolis JD, Sacco RE
Biochemical and biophysical research communications 2014 Mar 7;445(2):417-21
Biochemical and biophysical research communications 2014 Mar 7;445(2):417-21
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 20485-1-AP(TMEM165 antibody) at dilution of 1:3000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The TMEM165 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human TMEM165. This antibody recognizes human,mouse,rat antigen. The TMEM165 antibody has been validated for the following applications: ELISA, IF, IP, WB analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-TMEM165 (IP:20485-1-AP, 4ug; Detection:20485-1-AP 1:1000) with HeLa cells lysate 3440ug.
- Sample type
- cell line