11381-1-AP
antibody from Invitrogen Antibodies
Targeting: CTTN
EMS1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 11381-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cortactin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: VDKSAVGFE YQGKTEKHES QKDYVKGFGG KFGVQTDRQD KCALGWDHQE KLQLHESQKD YSKGFGGKYG VQKDRMDKNA STFEDVTQVS SAYQKTVPVE AVTSKTSNIR ANFENLAKEK EQEDRRKAEA ERAQRMAKER QEQEEARRKL EEQARAKTQT PPVSPAPQPT EERLPSSPVY EDAASFKAEL SYRGPVSGTE PEPVYSMEAA DYREASSQQG LAYATEAVYE SAEAPGHYPA EDSTYDEYEN DLGITAVALY DYQAAGDDEI SFDPDDIITN IEMIDDGWWR GVCKGRYGLF PANYVELRQ (206-513 aa encoded by BC008799)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.43 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HT-1080 cells were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 11381-1-AP (Cortactin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug Cortactin antibody (11381-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Cortactin (IP:11381-1-AP, 4ug; Detection:11381-1-AP 1:1000) with MCF-7 cells lysate 2400 ug.