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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- AF6096 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat Cortactin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and rat Cortactin in Western blots.
- Reactivity
- Human, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q14247- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human Cortactin Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cortactin knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Cortactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6096) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cortactin at approximately 80 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat Cortactin by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, MDA-MB-453 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Cortactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6096) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Cortactin at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
