12-9919-42

antibody from Invitrogen Antibodies

Targeting: FASLG APT1LG1, CD178, FasL, TNFSF6

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12-9919-42

Provider

Invitrogen Antibodies

Product name

CD178 (Fas Ligand) Monoclonal Antibody (NOK-1), PE, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The NOK-1 monoclonal antibody reacts with human Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by neutrophils, monocytes, and activated T cells and NK cells. The interaction of FasL with its receptor (CD95, Fas) induces Fas-mediated killing of lymphocytes. Human FasL is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. Therefore for optimal detection of surface FasL on activated peripheral blood cells, incubation of cells with an MMP inhibitor is recommended.

Conjugate

Yellow dye

Antibody clone number

NOK-1

Concentration

5 µL/Test

References

Submitted references

Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion.

Myers JA, Schirm D, Bendzick L, Hopps R, Selleck C, Hinderlie P, Felices M, Miller JS
JCI insight 2022 Aug 8;7(15)

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CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity.

Bernareggi D, Xie Q, Prager BC, Yun J, Cruz LS, Pham TV, Kim W, Lee X, Coffey M, Zalfa C, Azmoon P, Zhu H, Tamayo P, Rich JN, Kaufman DS
Nature communications 2022 Apr 7;13(1):1899

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Silencing of soluble epoxide hydrolase 2 gene reduces H(2)O(2)-induced oxidative damage in rat intestinal epithelial IEC-6 cells via activating PI3K/Akt/GSK3β signaling pathway.

Li J, Luo J, Zhang Y, Tang C, Wang J, Chen C
Cytotechnology 2020 Feb;72(1):23-36

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Interleukin-24 Regulates T Cell Activity in Patients With Colorectal Adenocarcinoma.

Zhang Y, Liu Y, Xu Y
Frontiers in oncology 2019;9:1401

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Transmembrane TNF-α promotes activation-induced cell death by forward and reverse signaling.

Zhang M, Wang J, Jia L, Huang J, He C, Hu F, Yuan L, Wang G, Yu M, Li Z
Oncotarget 2017 Sep 8;8(38):63799-63812

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IL12-mediated sensitizing of T-cell receptor-dependent and -independent tumor cell killing.

Braun M, Ress ML, Yoo YE, Scholz CJ, Eyrich M, Schlegel PG, Wölfl M
Oncoimmunology 2016 Jul;5(7):e1188245

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Long-term effect on natural killer cells by interferon-α therapy on the outcomes of HCV infection.

Hu X, Jiang Y, Li X, Gao Y, Guo X, Chi X, Yan H, Feng J, Zhong J, Sun B, Shao X, Xiao W, Pan Y, Niu J
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2014 May;34(5):366-75

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Fas-mediated killing of primary prostate cancer cells is increased by mitoxantrone and docetaxel.

Symes JC, Kurin M, Fleshner NE, Medin JA
Molecular cancer therapeutics 2008 Sep;7(9):3018-28

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Siva-1 negatively regulates NF-kappaB activity: effect on T-cell receptor-mediated activation-induced cell death (AICD).

Gudi R, Barkinge J, Hawkins S, Chu F, Manicassamy S, Sun Z, Duke-Cohan JS, Prasad KV
Oncogene 2006 Jun 8;25(24):3458-62

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c-FLIP inhibits chemotherapy-induced colorectal cancer cell death.

Longley DB, Wilson TR, McEwan M, Allen WL, McDermott U, Galligan L, Johnston PG
Oncogene 2006 Feb 9;25(6):838-48

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Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing.

Suda T, Hashimoto H, Tanaka M, Ochi T, Nagata S
The Journal of experimental medicine 1997 Dec 15;186(12):2045-50

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Expression of the functional soluble form of human fas ligand in activated lymphocytes.

Tanaka M, Suda T, Takahashi T, Nagata S
The EMBO journal 1995 Mar 15;14(6):1129-35

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Metalloproteinase-mediated release of human Fas ligand.

Kayagaki N, Kawasaki A, Ebata T, Ohmoto H, Ikeda S, Inoue S, Yoshino K, Okumura K, Yagita H
The Journal of experimental medicine 1995 Dec 1;182(6):1777-83

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Surface staining of human FasL tranfected cells with Anti-Human CD178 (CD95 Ligand) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Conjugate

Yellow dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Yellow dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EVs secreted by Cal27 induce apoptosis in NK cells. a Cal27-WT and KO derived EVs were analyzed by flow cytometry for known NKG2D ligands MICA/B, ULBP2/5/6, ULPB3, and for TRAIL and FasL, proteins involved in intrinsic or extrinsic apoptosis pathway (green histograms). In blue is shown the isotype control. Representative of n = 2 independent experiments. b Flow cytometry CASP3/7 viability assay showing increasing NK cell death once exposed to EVs from Cal27 in a time course of 4-hours. The error bars represent +-SEM across n = 3 replicates. Two-way ANOVA and Bonferroni's post hoc test was performed to determine statistical significance. Representative of n = 3 independent experiments. c 24 hour time course viability assay quantified using the IncuCyte real-time imaging system. N = 5 images/well taken for each of three replicates. The error bars represent +-SEM across n = 3 replicates. Statistical analysis was performed by two-way ANOVA and Bonferroni's post hoc multiple comparison test. Representative of n = 2 independent experiments. d 4 hour cytotoxicity assay using NK cells as effectors against Cal27-WT or KO as target cells. Here we show how Cal27-derived EVs reduce the killing in Cal27-KO. Error bars represent +-SEM across n = 3 replicates. Statistical analysis was performed by one-way ANOVA and Bonferroni's post hoc test (ns, not significant). e antibodies blocking MICA/B, TRAIL, or a combination of the two (aMICA/B and aTRAIL) limit the inhibitory effect of EV on NK ce

Conjugate

Yellow dye