- PA1-916 - Invitrogen Antibodies ATP2B3 antibody

Submitted references Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.

Lisek M, Ferenc B, Studzian M, Pulaski L, Guo F, Zylinska L, Boczek T
Frontiers in cellular neuroscience 2017;11:181

Mutation of plasma membrane Ca2+ ATPase isoform 3 in a family with X-linked congenital cerebellar ataxia impairs Ca2+ homeostasis.

Zanni G, Calì T, Kalscheuer VM, Ottolini D, Barresi S, Lebrun N, Montecchi-Palazzi L, Hu H, Chelly J, Bertini E, Brini M, Carafoli E
Proceedings of the National Academy of Sciences of the United States of America 2012 Sep 4;109(36):14514-9

Differential regulation of the apical plasma membrane Ca(2+) -ATPase by protein kinase A in parotid acinar cells.

Baggaley E, McLarnon S, Demeter I, Varga G, Bruce JI
The Journal of biological chemistry 2007 Dec 28;282(52):37678-93

Plasma membrane calcium pumps in mouse olfactory sensory neurons.

Weeraratne SD, Valentine M, Cusick M, Delay R, Van Houten JL
Chemical senses 2006 Oct;31(8):725-30

The plasma membrane Ca2+-ATPase isoform 4 is localized in lipid rafts of cerebellum synaptic plasma membranes.

Sepúlveda MR, Berrocal-Carrillo M, Gasset M, Mata AM
The Journal of biological chemistry 2006 Jan 6;281(1):447-53

Expression and immunolocalization of plasma membrane calcium ATPase isoforms in human corneal epithelium.

Talarico EF Jr, Kennedy BG, Marfurt CF, Loeffler KU, Mangini NJ
Molecular vision 2005 Mar 2;11:169-78

Regional distribution of Na,K-ATPase activity in porcine lens epithelium.

Tamiya S, Dean WL, Paterson CA, Delamere NA
Investigative ophthalmology & visual science 2003 Oct;44(10):4395-9

Regional distribution of Na,K-ATPase activity in porcine lens epithelium.

Tamiya S, Dean WL, Paterson CA, Delamere NA
Investigative ophthalmology & visual science 2003 Oct;44(10):4395-9

Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies.

Caride AJ, Filoteo AG, Enyedi A, Verma AK, Penniston JT
The Biochemical journal 1996 May 15;316 ( Pt 1):353-9

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase (Product # PA1-916) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase (Product # PA1-916) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

PA1-916

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