Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-1807 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1807, RRID:AB_10001372
- Product name
- Rabbit Polyclonal Prohibitin 2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Mitochondrial dysfunction and adipogenic reduction by prohibitin silencing in 3T3-L1 cells.
Sphingosine-1-phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration.
A repressive role for prohibitin in estrogen signaling.
Liu D, Lin Y, Kang T, Huang B, Xu W, Garcia-Barrio M, Olatinwo M, Matthews R, Chen YE, Thompson WE
PloS one 2012;7(3):e34315
PloS one 2012;7(3):e34315
Sphingosine-1-phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration.
Strub GM, Paillard M, Liang J, Gomez L, Allegood JC, Hait NC, Maceyka M, Price MM, Chen Q, Simpson DC, Kordula T, Milstien S, Lesnefsky EJ, Spiegel S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Feb;25(2):600-12
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Feb;25(2):600-12
A repressive role for prohibitin in estrogen signaling.
He B, Feng Q, Mukherjee A, Lonard DM, DeMayo FJ, Katzenellenbogen BS, Lydon JP, O'Malley BW
Molecular endocrinology (Baltimore, Md.) 2008 Feb;22(2):344-60
Molecular endocrinology (Baltimore, Md.) 2008 Feb;22(2):344-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Prohibitin 2 Antibody [NB100-1807] - Detection of Human REA by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-REA antibodies NB100-1807, another REA Ab, NB100-1808, and NB100-1809 each used for IP at 3 mcg/mg lysate. Immunoprecipitated REA was blotted using BL1706 (upper blot) or BL1707 (lower blot). Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Prohibitin 2 Antibody [NB100-1807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- REA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB