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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- TA500665 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500665, RRID:AB_2250163
- Product name
- Anti-LTA4H (Leukotriene A4 hydrolase) mouse monoclonal antibody, clone OTI8F4 (formerly 8F4)
- Antibody type
- Monoclonal
- Description
- Anti-LTA4H (Leukotriene A4 hydrolase) mouse monoclonal antibody, clone OTI8F4 (formerly 8F4)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LTA4H
- Isotype
- IgG
- Antibody clone number
- OTI8F4
- Vial size
- 100 µl
- Concentration
- 1.3 mg/ml
Submitted references Blocking macrophage leukotriene b4 prevents endothelial injury and reverses pulmonary hypertension.
Tian W, Jiang X, Tamosiuniene R, Sung YK, Qian J, Dhillon G, Gera L, Farkas L, Rabinovitch M, Zamanian RT, Inayathullah M, Fridlib M, Rajadas J, Peters-Golden M, Voelkel NF, Nicolls MR
Science translational medicine 2013 Aug 28;5(200):200ra117
Science translational medicine 2013 Aug 28;5(200):200ra117
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LTA4H monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LTA4H (RC207617, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LTA4H .
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunofluorescence of LTA4H protein level by using anti-LTA4H antibody in human lung tissues.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunofluorescence of LTA4H protein level by using anti-LTA4H antibody in human lung tissues.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of LTA4H by using TrueMab monoclonal anti-LTA4H antibody (Negative control: IP without adding anti-LTA4H antibody). For each experiment, 500ul of DDK tagged LTA4H overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-LTA4H mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500665, Dilution 1:50)
- Validation comment
- IHC
