PA5-35893

antibody from Invitrogen Antibodies

Targeting: H2AC11 H2A.1b, H2A/p, H2AFP, HIST1H2AG, pH2A/f

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35893

Provider

Invitrogen Antibodies

Product name

Histone H2A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: MCF-7, MDA-MB-231.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Histone H2A using A) 30 µg MCF-7 whole cell extract and B) 30 µg MDA-MB-231 whole cell extract. Samples were loaded onto a 15% SDS-PAGE gel and probed with a Histone H2A polyclonal antibody (Product # PA5-35893) at a dilution of 1:10000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Histone H2A Polyclonal Antibody (Product # PA5-35893). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A Polyclonal Antibody (Product # PA5-35893) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Histone H2A Polyclonal Antibody detects Histone H2A protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Histone H2A Polyclonal Antibody (Product # PA5-35893) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Histone H2A Polyclonal Antibody detects Histone H2A protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H2A Polyclonal Antibody (Product # PA5-35893) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Histone H2A Polyclonal Antibody detects Histone H2A protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H2A Polyclonal Antibody (Product # PA5-35893) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Histone H2A Polyclonal Antibody detects Histone H2A protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Histone H2A Polyclonal Antibody (Product # PA5-35893) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.