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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-46845 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- B-Myb Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: RCEDLDELHY QDTDSDVPEQ RDSKCKVKWT HEEDEQLRAL VRQFGQQDWK
- Concentration
- 0.5 mg/mL
Submitted references Global targetome analysis reveals critical role of miR-29a in pancreatic stellate cell mediated regulation of PDAC tumor microenvironment.
Dey S, Liu S, Factora TD, Taleb S, Riverahernandez P, Udari L, Zhong X, Wan J, Kota J
BMC cancer 2020 Jul 13;20(1):651
BMC cancer 2020 Jul 13;20(1):651
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HepG2 cell lysate using an anti-B-Myb polyclonal antibody (Product # PA5-46845).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human spermatophore tissue using an anti-B-Myb polyclonal antibody (Product # PA5-46845).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Validation of miR-29a direct target. a Relative fold changes estimated by qPCR analysis for the top miR-29a candidate target genes of ITGA6, ADAMTS2, IGF-1, COL5A3, CLDN1, E2F7 and MYBL2 in hPSCs transfected with miR-29a mimics (29a OE) compared with cells transfected with scramble control (CTRL). Numerical data are represented as average fold change (DeltaDeltaCT) +- standard error of the mean (SEM); ** p < 0.01; n = 3. b Total protein harvested from the hPSCs transfected with scramble control (CTRL) or miR-29a mimics (29a OE) 48 h post-transfection were subjected to western blot analysis for miR-29a candidate targets of ITGA6, ADAMTS2, IGF-1, COL5A3, CLDN1, E2F7 and MYBL2. GAPDH was used as the loading control. Quantification of band intensities normalized to GAPDH. Quantification of band intensities normalized to GAPDH and relative to respective controls are represented as +- SEM; n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001 (right). Uncropped blots are shown in Additional file 3 : Fig. S1
