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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX109503 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109503, RRID:AB_11173871
- Product name
- GCSF Receptor antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- GCSF Receptor antibody [N2C1], Internal detects CSF3R protein by western blot analysis.A. 30 ?g THP-1 whole cell lysate/extract5% SDS-PAGEGCSF Receptor antibody [N2C1], Internal (GTX109503) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GCSF Receptor antibody detects GCSF Receptor protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GCSF Receptor antibody (GTX109503) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GCSF Receptor antibody [N2C1], Internal detects CSF3R protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CSF3R protein stained by GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GCSF Receptor antibody [N2C1], Internal detects GCSF Receptor protein at secreted on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:250.
