Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- ABIN499331 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- A 19 amino acid peptide from near the carboxy terminus of Human ARF-BP1 (AP30078CP-N)
- Description
- Affinity chromatography purified via peptide column
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Epitope
- C-Term
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references The ARF tumour suppressor.
Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis.
ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor.
Gallagher SJ, Kefford RF, Rizos H
The international journal of biochemistry & cell biology 2006;38(10):1637-41
The international journal of biochemistry & cell biology 2006;38(10):1637-41
Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis.
Zhong Q, Gao W, Du F, Wang X
Cell 2005 Jul 1;121(7):1085-95
Cell 2005 Jul 1;121(7):1085-95
ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor.
Chen D, Kon N, Li M, Zhang W, Qin J, Gu W
Cell 2005 Jul 1;121(7):1071-83
Cell 2005 Jul 1;121(7):1071-83
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ?g/ml.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunocytochemistry of ARF-BP1 in Daudi cells with AP30078PU-N ARF-BP1 antibody at 5 ?g/ml.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunofluorescence of ARF-BP1 in Daudi cells with AP30078PU-N ARF-BP1 antibody at 20 ?g/ml.