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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-78398 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MOG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: mouse brain, rat brain
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MOG in various tissue extracts using 50 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with MOG polyclonal antibody (Product # PA5-78398) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MOG Polyclonal Antibody (Product # PA5-78398). Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MOG Polyclonal Antibody (Product # PA5-78398) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MOG Polyclonal Antibody detects MOG protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MOG protein stained by MOG Polyclonal Antibody (Product # PA5-78398) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MOG Polyclonal Antibody detects MOG protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: MOG protein stained by MOG Polyclonal Antibody (Product # PA5-78398) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI .
