Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB5674 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal L1CAM Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse L1CAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM, rhNCAM-L1, rhOBCAM, recombinant mouse (rm)MAdCAM-1, or rmOCAM is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Promotion of spinal cord regeneration by neural stem cell-secreted trimerized cell adhesion molecule L1.
He X, Knepper M, Ding C, Li J, Castro S, Siddiqui M, Schachner M
PloS one 2012;7(9):e46223
PloS one 2012;7(9):e46223
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of L1CAM in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse L1CAM Monoclonal Antibody (Catalog # MAB5674, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).