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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX84244 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84244, RRID:AB_10729891
- Product name
- L1CAM antibody [1H3]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-L1CAM mouse monoclonal antibody (GTX84244) immunofluorescent staining of COS7 cells transiently transfected with L1CAM
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either L1CAM overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-L1CAM antibody (GTX84244), and then analyzed by flow cytometry.
