TA501441

antibody from OriGene

Targeting: L1CAM CD171, HSAS, HSAS1, MASA, MIC5, S10, SPG1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501441

Provider

OriGene

Proper citation

OriGene Cat#TA501441, RRID:AB_11126060

Product name

Anti-L1CAM mouse monoclonal antibody, clone OTI2G9 (formerly 2G9)

Antibody type

Monoclonal

Description

Anti-L1CAM mouse monoclonal antibody, clone OTI2G9 (formerly 2G9)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

L1CAM

Isotype

IgG

Antibody clone number

OTI2G9

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-L1CAM mouse monoclonal antibody (TA501441) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM(RC211601).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC211601 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-L1CAM antibody(TA501441), and then analyzed by flow cytometry.

Validation comment

FC