Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB5744 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Galectin-14 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Galectin-14 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Galectin-1, -2, -3, -4, -7, -8, -10, or recombinant mouse Galectin-9 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8TCE9
- Isotype
- IgG
- Antibody clone number
- 511124
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease.
Bolnick JM, Kohan-Ghadr HR, Fritz R, Bolnick AD, Kilburn BA, Diamond MP, Armant DR, Drewlo S
Scientific reports 2016 Sep 23;6:32382
Scientific reports 2016 Sep 23;6:32382
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Galectin-14 by Western Blot. Western blot shows lysates of human eosinophil. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Galectin-14 Monoclonal Antibody (Catalog # MAB5744) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Galectin-14 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.