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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA500904 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500904, RRID:AB_11125293
- Product name
- CISD1 mouse monoclonal antibody, clone OTI2B3 (formerly 2B3)
- Antibody type
- Monoclonal
- Description
- CISD1 mouse monoclonal antibody, clone OTI2B3 (formerly 2B3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CISD1
- Isotype
- IgG
- Antibody clone number
- OTI2B3
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (RC203308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-CISD1 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-CISD1 mouse monoclonal antibody (TA500904) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1(RC203308).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-CISD1 mouse monoclonal antibody (TA500904).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-CISD1 antibody(TA500904),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
