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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- TA500905 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500905, RRID:AB_11125393
- Product name
- Anti-CISD1 mouse monoclonal antibody, clone OTI3A1 (formerly 3A1)
- Antibody type
- Monoclonal
- Description
- Anti-CISD1 mouse monoclonal antibody, clone OTI3A1 (formerly 3A1)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CISD1
- Isotype
- IgG
- Antibody clone number
- OTI3A1
- Vial size
- 100 µl
- Concentration
- 0.76 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (RC203308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-CISD1 mouse monoclonal antibody (TA500905) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1(RC203308).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-CISD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500905, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CISD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500905, Dilution 1:50)
- Validation comment
- IHC
