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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP21841PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21841PU-N, RRID:AB_10756421
- Product name
- anti LRRC32 / GARP
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 250-300 of Human GARP/LRRC32
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of GARP/LRRC32 in mouse thymus lysate in the 1) absence and 2) presence of immunizing peptide using AP21841PU-N at 0.5 µg/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- BALB/c splenocytes stained with anti-GARP, with a FITC conjugated secondary, and anti-mouse CD4 PE. Viable cells in the lymphocyte gate were used for analysis. Cell surface flow kit was used for this test (cells were not fixed for testing).
