PA5-16519

antibody from Invitrogen Antibodies

Targeting: CCNA1 CT146

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-16519

Provider

Invitrogen Antibodies

Product name

Cyclin A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA5-16519 targets Cyclin A in IHC (P), IP, and WB applications and shows reactivity with mouse, Rat, and Human samples.

Concentration

1 mg/mL

References

Submitted references

Long Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway.

Li X, Wang H
Neuropsychiatric disease and treatment 2021;17:2073-2087

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Effect of long non-coding RNA PVT1 on cell proliferation and migration in melanoma.

Chen L, Ma D, Li Y, Li X, Zhao L, Zhang J, Song Y
International journal of molecular medicine 2018 Mar;41(3):1275-1282

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B-Myb enhances proliferation and suppresses differentiation of keratinocytes in three-dimensional cell culture.

Maruyama H, Ishitsuka Y, Fujisawa Y, Furuta J, Sekido M, Kawachi Y
Archives of dermatological research 2014 May;306(4):375-84

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Pituitary tumor transforming gene 1 induces tumor necrosis factor-α production from keratinocytes: implication for involvement in the pathophysiology of psoriasis.

Ishitsuka Y, Kawachi Y, Maruyama H, Taguchi S, Fujisawa Y, Furuta J, Nakamura Y, Ishii Y, Otsuka F
The Journal of investigative dermatology 2013 Nov;133(11):2566-2575

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Expression of cyclin A, cyclin E and p27 in normal, hyperplastic and frankly malignant endometrial samples.

Gezginc ST, Celik C, Dogan NU, Toy H, Tazegul A, Colakoglu MC
Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology 2013 Jul;33(5):508-11

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of Cyclin A using Cyclin A Polyclonal Antibody (Product # PA5-16519) on HeLa Cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human tonsil stained with Cyclin A antibody using peroxidase-conjugate and AEC chromogen. Note nuclear and cytoplasmic staining of cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Cyclin A using Cyclin A Polyclonal Antibody (Product # PA5-16519) on denatured Human HeLa Cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 ZNF367 activates the cell cycle signaling pathway to promote glioma progression. ( A and B ) a KEGG analysis based on the genes showing a strong relevance (correlation coefficient R > 0.05) to ZNF367 in the TCGA-GBM database; ( C ) correlations between GABPB1-AS1 and the cell cycle-related factors PCNA, CDC20, CDC7 and CCNA1 predicted in the TCGA-GBM database; ( D and E ) protein levels of PCNA, CDC20, CDC7 and CCNA1 in U251 and SHG44 cells examined by Western blot analysis. Data were collected from three independent experiments and expressed as the mean +- SD. Data were analyzed by two-way ANOVA. ** p < 0.01 vs NC group, ## p < 0.01 vs shRNA + Inhibitor NC group. Abbreviations: NC, negative control; shRNA, short hairpin RNA of GABPB1-AS1; PCNA, proliferating cell nuclear antigen; CCNA1, cyclin A1.