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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB1887 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1887, RRID:AB_1577575
- Product name
- NANOG polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NANOG.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Nanog inhibits the switch of myogenic cells towards the osteogenic lineage.
Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract.
Kochupurakkal BS, Sarig R, Fuchs O, Piestun D, Rechavi G, Givol D
Biochemical and biophysical research communications 2008 Jan 25;365(4):846-50
Biochemical and biophysical research communications 2008 Jan 25;365(4):846-50
Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract.
Freberg CT, Dahl JA, Timoskainen S, Collas P
Molecular biology of the cell 2007 May;18(5):1543-53
Molecular biology of the cell 2007 May;18(5):1543-53
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NANOG polyclonal antibody (Cat # PAB1887) in HeLa cell line lysates (35 ug/lane). NANOG (arrow) was detected using the purified polyclonal antibody.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NANOG (arrow) using NANOG polyclonal antibody (Cat # PAB1887). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NANOG polyclonal antibody (Cat # PAB1887) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue reacted with NANOG polyclonal antibody (Cat # PAB1887) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using NANOG polyclonal antibody (Cat # PAB1887)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
