GTX627421

antibody from GeneTex

Targeting: NANOG FLJ12581, FLJ40451

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX627421

Provider

GeneTex

Proper citation

GeneTex Cat#GTX627421, RRID:AB_11169335

Product name

Nanog antibody [GT3312]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

References

Submitted references

The antipsychotic chlorpromazine suppresses YAP signaling, stemness properties, and drug resistance in breast cancer cells.

Yang CE, Lee WY, Cheng HW, Chung CH, Mi FL, Lin CW
Chemico-biological interactions 2019 Apr 1;302:28-35

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Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.

Fan JR, Lee HT, Lee W, Lin CH, Hsu CY, Hsieh CH, Shyu WC
Cell death & disease 2018 May 1;9(5):502

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Thyroid hormone negatively regulates tumorigenesis through suppression of BC200.

Lin YH, Wu MH, Huang YH, Yeh CT, Chi HC, Tsai CY, Chuang WY, Yu CJ, Chung IH, Chen CY, Lin KH
Endocrine-related cancer 2018 Dec 1;25(12):967-979

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MDM2 Degrades Deacetylated Nucleolin Through Ubiquitination to Promote Glioma Stem-Like Cell Enrichment for Chemotherapeutic Resistance.

Ko CY, Lin CH, Chuang JY, Chang WC, Hsu TI
Molecular neurobiology 2018 Apr;55(4):3211-3223

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Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.

Chang KY, Huang CT, Hsu TI, Hsu CC, Liu JJ, Chuang CK, Hung JJ, Chang WC, Tsai KK, Chuang JY
Biochemical and biophysical research communications 2017 Nov 4;493(1):14-19

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Stress granule-associated protein G3BP2 regulates breast tumor initiation.

Gupta N, Badeaux M, Liu Y, Naxerova K, Sgroi D, Munn LL, Jain RK, Garkavtsev I
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 31;114(5):1033-1038

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The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.

Huang TT, Ping YH, Wang AM, Ke CC, Fang WL, Huang KH, Lee HC, Chi CW, Yeh TS
Oncotarget 2015 Jul 20;6(20):18012-26

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Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis.

Wang AM, Huang TT, Hsu KW, Huang KH, Fang WL, Yang MH, Lo SS, Chi CW, Lin JJ, Yeh TS
Oncotarget 2014 Jul 15;5(13):5002-16

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CD164 regulates the tumorigenesis of ovarian surface epithelial cells through the SDF-1α/CXCR4 axis.

Huang AF, Chen MW, Huang SM, Kao CL, Lai HC, Chan JY
Molecular cancer 2013 Oct 5;12(1):115

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Ovarian cancer stem-like cells show induced translineage-differentiation capacity and are suppressed by alkaline phosphatase inhibitor.

Liu KC, Yo YT, Huang RL, Wang YC, Liao YP, Huang TS, Chao TK, Lin CK, Weng SJ, Ma KH, Chang CC, Yu MH, Lai HC
Oncotarget 2013 Dec;4(12):2366-82

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

NANOG antibody [GT3312] validation by siRNA knock-down.Upperpanel: NANOG antibody [GT3312] GTX627421Lower panel: GAPDH antibody (GTX100118)A. 30 ?g Tera-2 whole cell lysate/extract B. 30 ?g whole cell lysate/extract of NANOG siRNA#1-transfected Tera-2 cells C. 30 ?g whole cell lysate/extract of NANOG siRNA#2-transfected Tera-2 cells D. 30 ?g whole cell lysate/extract of NANOG siRNA#3-transfected Tera-2 cells 10% SDS-PAGENANOG antibody [GT3312] (GTX627421) dilution: 1:1000GAPDH antibody (GTX100118) dilution: 1:10000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

<50%

KD Reagent Provider

Customized siRNA

KD Reagent Prod no

GTX627421

KD Reagent Prod page

http://www.genetex.com

Antibody Lot Number

40878

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE GTX627421 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NANOG antibody [GT3312] detects NANOG protein by western blot analysis.A. 30 ?g human ESC whole cell lysate/extract10% SDS-PAGENANOG antibody [GT3312] (GTX627421) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (20 ?g of whole cell lysate) A: mouse ESC 10% SDS PAGE GTX627421 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NANOG antibody [GT3312] detects NANOG protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: NANOG antibody [GT3312] dilution: 1:50