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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX109159 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109159, RRID:AB_1949730
- Product name
- BCL10 antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample(30 ug whole cell lysate)A:Hep G2 (GTX27900)B:MOLT4 (GTX27912)C:Raji (GTX27908)12% SDS PAGEGTX109159 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE BCL10 antibody GTX109159 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BCL10 antibody [N1C3] immunoprecipitates BCL10 protein in IP experiments.IP samples: Raji whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of BCL10 protein by 4 £gg BCL10 antibody [N1C3] (GTX109159)12 % SDS-PAGEThe immunoprecipitated BCL10 protein was detected by BCL10 antibody [N1C3] (GTX109159) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BCL10 antibody [N1C3] detects BCL10 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. BCL10 antibody [N1C3] (GTX109159) diluted at 1:500.
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- GeneTex (provider)
- Main image
- Experimental details
- BCL10 antibody [N1C3] detects BCL10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. BCL10 antibody [N1C3] (GTX109159) diluted at 1:500.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with MCF-7 chromatin extract treated with TNF-a (10 ng/ml for 30 min) and 5 £gg of either control rabbit IgG or anti-BCL10 antibody. The precipitated DNA was detected by PCR with primer set targeting to I£eB£\.
