Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX112744 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112744, RRID:AB_11176414
- Product name
- BCL10 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE GTX112744 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BCL10 antibody detects BCL10 protein by Western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extract C. 30 ?g C8D30 whole cell lysate/extract12 % SDS-PAGEBCL10 antibody (GTX112744) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and BCL10 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL10 antibody (GTX112744) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and BCL10 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL10 antibody (GTX112744) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BCL10 antibody detects BCL10 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: BCL10 protein stained by BCL10 antibody (GTX112744) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BCL10 antibody immunoprecipitates BCL10 protein in IP experiments.IP samples: Raji whole cell extractA. 40 £gg Raji whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of BCL10 protein by 4 £gg BCL10 antibody (GTX112744)12 % SDS-PAGEThe immunoprecipitated BCL10 protein was detected by BCL10 antibody (GTX112744) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using BCL10 antibody(GTX112744) antibody at 1:500 dilution.
