- GTX70111 - GeneTex BRCA1 antibody

Submitted references The Rb binding domain of HPV31 E7 is required to maintain high levels of DNA repair factors in infected cells.

Johnson BA, Aloor HL, Moody CA
Virology 2017 Jan;500:22-34

Differences in the recruitment of DNA repair proteins at subtelomeric and interstitial I-SceI endonuclease-induced DNA double-strand breaks.

Alcaraz Silva B, Jones TJ, Murnane JP
DNA repair 2017 Jan;49:1-8

Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells.

Shang HS, Chang CH, Chou YR, Yeh MY, Au MK, Lu HF, Chu YL, Chou HM, Chou HC, Shih YL, Chung JG
Oncology reports 2016 Oct;36(4):2207-15

Casticin induces DNA damage and inhibits DNA repair-associated protein expression in B16F10 mouse melanoma cancer cells.

Shih YL, Chou J, Yeh MY, Chou HM, Chou HC, Lu HF, Shang HS, Chueh FS, Chu YL, Hsueh SC, Chung JG
Oncology reports 2016 Oct;36(4):2094-100

Histone H2B-IFI16 Recognition of Nuclear Herpesviral Genome Induces Cytoplasmic Interferon-β Responses.

Iqbal J, Ansari MA, Kumar B, Dutta D, Roy A, Chikoti L, Pisano G, Dutta S, Vahedi S, Veettil MV, Chandran B
PLoS pathogens 2016 Oct;12(10):e1005967

RECQ5 helicase promotes resolution of conflicts between replication and transcription in human cells.

Urban V, Dobrovolna J, Hühn D, Fryzelkova J, Bartek J, Janscak P
The Journal of cell biology 2016 Aug 15;214(4):401-15

BRCA1 Regulates IFI16 Mediated Nuclear Innate Sensing of Herpes Viral DNA and Subsequent Induction of the Innate Inflammasome and Interferon-β Responses.

Dutta D, Dutta S, Veettil MV, Roy A, Ansari MA, Iqbal J, Chikoti L, Kumar B, Johnson KE, Chandran B
PLoS pathogens 2015 Jun;11(6):e1005030

PALB2 self-interaction controls homologous recombination.

Buisson R, Masson JY
Nucleic acids research 2012 Nov 1;40(20):10312-23

Can soy phytoestrogens decrease DNA methylation in BRCA1 and BRCA2 oncosuppressor genes in breast cancer?

Bosviel R, Dumollard E, Déchelotte P, Bignon YJ, Bernard-Gallon D
Omics : a journal of integrative biology 2012 May;16(5):235-44

Soy phytoestrogens modify DNA methylation of GSTP1, RASSF1A, EPH2 and BRCA1 promoter in prostate cancer cells.

Vardi A, Bosviel R, Rabiau N, Adjakly M, Satih S, Dechelotte P, Boiteux JP, Fontana L, Bignon YJ, Guy L, Bernard-Gallon DJ
In vivo (Athens, Greece) 2010 Jul-Aug;24(4):393-400

An hGCN5/TRRAP histone acetyltransferase complex co-activates BRCA1 transactivation function through histone modification.

Oishi H, Kitagawa H, Wada O, Takezawa S, Tora L, Kouzu-Fujita M, Takada I, Yano T, Yanagisawa J, Kato S
The Journal of biological chemistry 2006 Jan 6;281(1):20-6

Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia.

Bernard-Gallon DJ, Dechelotte PJ, Le Corre L, Chalabi N, Vissac-Sabatier C, Bignon YJ
Anticancer research 2004 Jan-Feb;24(1):321-4

BRCA1 function mediates a TRAP/DRIP complex through direct interaction with TRAP220.

Wada O, Oishi H, Takada I, Yanagisawa J, Yano T, Kato S
Oncogene 2004 Aug 5;23(35):6000-5

BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest.

Ongusaha PP, Ouchi T, Kim KT, Nytko E, Kwak JC, Duda RB, Deng CX, Lee SW
Oncogene 2003 Jun 12;22(24):3749-58

BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.

Bernard-Gallon DJ, Déchelotte P, Vissac C, Aunoble B, Cravello L, Malpuech G, Bignon YJ
Breast cancer research : BCR 2001;3(1):61-5

Tumor suppressor p53 is required to modulate BRCA1 expression.

Arizti P, Fang L, Park I, Yin Y, Solomon E, Ouchi T, Aaronson SA, Lee SW
Molecular and cellular biology 2000 Oct;20(20):7450-9

Transactivation of the p21 promoter by BRCA1 splice variants in mammary epithelial cells: evidence for both common and distinct activities of wildtype and mutant forms.

Lu M, Arrick BA
Oncogene 2000 Dec 14;19(54):6351-60

ATM complexes with HDM2 and promotes its rapid phosphorylation in a p53-independent manner in normal and tumor human cells exposed to ionizing radiation.

de Toledo SM, Azzam EI, Dahlberg WK, Gooding TB, Little JB
Oncogene 2000 Dec 14;19(54):6185-93

Heregulin induces phosphorylation of BRCA1 through phosphatidylinositol 3-Kinase/AKT in breast cancer cells.

Altiok S, Batt D, Altiok N, Papautsky A, Downward J, Roberts TM, Avraham H
The Journal of biological chemistry 1999 Nov 5;274(45):32274-8

BRCA1 and BRCA2 proteins are expressed in milk fat globules.

Bernard-Gallon DJ, Dechelotte P, Rio PG, Bignon YJ
International journal of cancer 1999 May 31;81(5):839-43

Reduction of BRCA1 protein expression in Japanese sporadic breast carcinomas and its frequent loss in BRCA1-associated cases.

Yoshikawa K, Honda K, Inamoto T, Shinohara H, Yamauchi A, Suga K, Okuyama T, Shimada T, Kodama H, Noguchi S, Gazdar AF, Yamaoka Y, Takahashi R
Clinical cancer research : an official journal of the American Association for Cancer Research 1999 Jun;5(6):1249-61

Expression of human BRCA1 and BRCA2 proteins in lung from a fetus at 19 weeks' gestation.

Bernard-Gallon DJ, Dechelotte P, Rio PG, Bignon YJ
International journal of cancer 1999 Aug 27;82(5):771-3

No comments: Submit comment

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (60 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 41729

: Show more

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: BRCA1 antibody 17F8 (GTX70111) was used for WB at 1:1000 dilution. MCF7 (human) and MEF(mouse embryonic fibroblast) cell lysate were used in the Western blot assay with a 5-15% gradient gel.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: 293T B: HeLa 5% SDS PAGE GTX70111 diluted at 1:500
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (60 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.

GTX70111

Antibody data