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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MAB7157 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal DTX1 Antibody
- Antibody type
- Monoclonal
- Antigen
- E. coli-derived recombinant human DTX1 Met1-Phe147 Accession # Q86Y01
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human DTX1/DTX4 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DTX1/DTX4 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of DTX1/DTX4 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
